摘要
Using a synthetic oligonucleotide （dA-dC）<sub>15</sub> as a probe to screen a probe pool made by microdissected human chromosome band 17q11-q12, a DNA fragment containing （CA）<sub>16</sub> was isolated, which is a novel short tandem repeat （STR） determined by searching in the GenBank and GDB. It has 8 allelic types with a PIC value of 0.61. The novel STR is conformed with Mendelian segregation according to the linkage analysis of a three-generation neurofibromatosis type Ⅰ （NF1） pedigree. The STR was localized at chromosome band 17q11-q12 in the vicinity of NF1 gene by FISH analysis. The accession number of the STR in GenBank and GDB is G32112 and D17S2204 respectively.
Using a synthetic oligonucleotide (dA-dC)15 as a probe to screen a probe pool made by microdissected human chromosome band 17q11-q12, a DNA fragment containing (CA)16 was isolated, which is a novel short tandem repeat (STR) determined by searching in the GenBank and GDB. It has 8 allelic types with a PIC value of 0.61. The novel STR is conformed with Mendelian segregation according to the linkage analysis of a three-generation neurofibromatosis type I (NF1) pedigree. The STR was localized at chromosome band 17q11-q12 in the vicinity of NFl gene by FISH analysis. The accession number of the STR in GenBank and GDB is G32112 and Dl752204 respectively.
