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Monitoring gene expression by cDNA array 被引量：3

Monitoring gene expression by cDNA array
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摘要 A new method designated cDNA array was developed by hybridization of quantitatively arrayed DNA samples isolated randomly from a cDNA library with probes reverse-transcribed from mRNAs of different sources or treatments. The gene expression patterns of 1 000 randomly chosen clones from an Arabidopsis library were analyzed with green seedlings versus suspension cells and seedlings irradiated under UV light. Northern blot and sequence analysis of some differentially expressed clones confirmed the results revealed by cDNA array, indicating that this method is efficient and reliable to monitor gene expression. A new method designated cDNA array was developed by hybridization of quantitatively arrayed DNA samples isolated randomly from a cDNA library with probes reverse-transcribed from mRNAs of different sources or treatments. The gene expression patterns of 1 000 randomly chosen clones from an Arabidopsis library were analyzed with green seedlings versus suspension cells and seedlings irradiated under UV light. Northern blot and sequence analysis of some differentially expressed clones confirmed the results revealed by cDNA array, indicating that this method is efficient and reliable to monitor gene expression.
作者 Yuxin Hu Chang Han Zhonglin Mou Jiayang Li
机构地区 Chinese Acad Sci DeMontfort Univ
出处 《Chinese Science Bulletin》 SCIE EI CAS 1999年第5期441-444,共4页
关键词 gene expression CDNA array FUNCTIONAL GENOMICS ARABIDOPSIS thaliana. gene expression cDNA array functional genomics Arabidopis thaliana
分类号 Q786 [生物学—分子生物学]
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参考文献15

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同被引文献17

  • 1胡玉欣,汪政科,王永红,包方,李凝,彭镇华,李家洋.Identification of brassinosteroid responsive genes in Arabidopsis by cDNA array[J].Science China(Life Sciences),2001,44(6):637-643. 被引量：4
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引证文献3

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Chinese Science Bulletin
1999年 第5期

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