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兔角膜上皮特异性角蛋白的电泳分析

Analysis of Specific Keratin Proteins by Electrophoreatic Technique
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摘要 本文提取兔角膜角蛋白,利用SDS-PAGE以及IEF/SDS-PAGE技术对其组成进行分析研究。结果表明,兔角幕上皮存在四种含量较高的特异性角蛋白,其分子量和等电点分别为:(Mr68kd,pI7.6);(Mr64kd,PI5.4);(Mr58kd,PI7.4);(Mr55kd,PI5.2). taking the comeal epithelium and skinny epiderm of rabbit and removing water -soluble proteins from them, the keratin proteins were extracted. The composition of the protein was analyzed by SDS -PAGE and IEF/SDS -PAGE. The results are as follows: four specific keratin proteins are pre sent in the rabbit corneal epithelium, and their molecular weights and isoelectric points are Mr 68 kd, pI7.6 Mr64 kd, pI5.4; Mr 58kd, pI.4; Mr55kd, PI 5.2
出处 《惠州学院学报》 1999年第4期32-35,共4页 Journal of Huizhou University
关键词 兔角膜上皮 特异性蛋白质 角蛋白 Comeal epithelium of rabbit, Keratin protein, Specific protein
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  • 1顾肃敏,仝允栩.花背蟾蜍角膜早期形态发生的超微结构研究[J]实验生物学报,1986(03).

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