树鼩实验感染丁型肝炎病毒后肝内抗原表达状态

INTRAHEPATIC EXPRESSION OF VIRAL ANTIGENS IN CHINESE TUPAIAE FOLLOWING HDV/HBV COINFECTION OR SUPERINFECTION

目的 研究树鼩实验感染人丁型肝炎病毒的可能性。方法 用免疫组化方法,检测树鼩肝组织丁型肝炎病毒抗原(HDAg),乙型肝炎病毒表面抗原HBsAg),采用原位杂交方法检测部分动物肝内HDV RNA。结果 35只动物,HDV/HBV同时感染组和重叠感染组HDAg阳性率分别为94.5％(21╱22)和84.6％(11/13);HDAg在肝内主要定位于肝细胞,在肝小叶呈灶状或片状分布,肝内持续时间在同时感染组和重叠感染组稍有不同,前者多持续3～4周,后者则可长达12周以上;部分动物肝组织细胞浆或细胞核HDV RNA阳性。结论 人HDV可感染树鼩,树鼩可成为丁型肝炎研究的动物模型。

Objectives To estabolish an animal infection model of hepatitis delta virus in adult Chinese Tupa-iae, intrahepatic expression of viral antigens in experimental HDV infected Tupaiae was studied. Methods Infection of tupaiae were performed by intraperitonal injection of 0. 2ml sera from HBV and HDVmarker positive patients. Thirty-five tupaiaes were divided into HDV/HBV coinfection group and HDV/HBV superinfection group. Animal sera and liver tissues were weekly obtained after inoculation. Liver tissues were studied with histopathological ,im-munohistochemical and in situ hybridizational methods. Rusults In HDV/HBV coinfection group,HDAg was detected in twenty one out of twenty -two tupaiaes,and HBsAg in fourteen of twenty; In HDV/HBV superinfection group,HDAg was positive in eleven of thirteen,and HBsAg in five of five detected. Intrahepatic HDAg expression oc-cured 2 - 3 weeks after inoculation,and it lasted for about 3-4 weeks in coinfection group, while 12wks in superinfection group. HDAg positive signales were localized in liver cell nuclei and cytoplasma .Conclusion Tupaia can be infected by inoculation of human HDAg positive sera.