儿茶素单体EGCG体外诱导胃癌MGC—803细胞凋亡的研究

Induction of apoptosis in human gastric cancer cell line by EGCG

目的：探讨表没食子儿茶素酸酯（Epigallatechin—3—gallate，EGCG）体外诱导胃癌MGC—803细胞凋亡作用。方法：以不同浓度EGCG分别处理MGC—803细胞，用琼脂糖凝胶电泳、荧光显微镜、MTT法观察MGC—803细胞DNA带型、形态和抑制率的变化。结果：琼脂糖凝胶电泳呈典型的DNA“梯状带”，高浓度EGCG（1×10-3M）作用33小时后DNA呈弥散的“膜状带”。荧光显微镜下可见细胞出现染色质浓缩、胞膜吐泡等凋亡现象。MTT法测定不同浓度EGCG对MGC—803细胞生长有明显抑制。结论：EGCG有明显的诱导胃癌MGC—803细胞凋亡作用，高浓度EGCG直接导致细胞坏死。

objective: To study the effect of epigallatechin-3-gallate (EGCG) on the growthand apoptosis of gastric cancer MGC-803 cell line. Methods: Changes in DNA fragment, morphol-ogy and growth inhibition rate of MGC- 803 cell line were evaluated by agrose gel eletrophoresis,fluorescent microscopy and MTT assay. Results:Apoptosis characterization of DNA laddering bandappeared in the MGC- 803 cell after treated with EGCG at 1×10-4 M or 1×10-5 M for 33 hours,but at 1×10-3M DNA fragment was smear band shaped. Condensation of chromosome and bleb-bing of the plasma membrane were found in the apoptotic cells under fluorescent microscopy. MTTassay showed growth inhibition of MGC- 803 cells induced by EGCG. Conclusion: EGCG may in-duce apoptosis of gastric cancer MGC-803 cells significantly, and high concentration EGCG maycause the necrosis of the cells directly.