中药肾衰宁对IL—1刺激后体外培养的人肾小球系膜细胞增殖的影响

Effect of Shenshuining on Proliferation of Human Glomerulor Mesangial Cells Cultured in Vitro Stimulated by Interleukin 1

目的：观察了具有降逆泄浊、益气活血作用的中药复方制剂肾衰宁灌肠液（以下简称肾衰宁，SSN），对IL—1刺激后体外培养的人肾小球系膜细胞增殖的影响，冀以从细胞生物学水平探讨肾衰宁防治慢性肾功能衰竭（CRF）的作用机制。方法：按经典方法培养、鉴定人肾小球系膜细胞，分五组，分别加入不同刺激物。按MTT比色法，用酶标仪测定吸光度，记录OD值并计算抑制率。结果：IL—1可明显刺激人肾小球系膜细胞增殖，SSN大鼠血清对IL—1刺激后人肾小球系膜细胞增殖有明显的抑制作用，其抑制程度与药物浓度有一定的量效关系、即随药物浓度的增加对系膜细胞增殖所产生的抑制作用也相应增加。结论：含肾衰宁的大鼠血清对IL—1刺激后肾小球系膜细胞的增殖有明显的抑制作用。提示肾小球系膜细胞是肾衰宁发挥治疗作用的重要靶细胞。

Objectly: It was observed that the effact of traditional chinese drugsShenshuining (SSN) which have the function of downbearing the reverse and discharging the turbid, tonfingQi and activating blood on proliferation of human glomerulor mesangial cells cultured in vitro bystimulated IL-1. The aim of this study was to approach the mechanism of SSN on prevention andtreatment of chronic renal failure (CRF) in level of cell biology. Methods: Acording to the classicalmethod huMsC were cultured and identificated. Then, it were classified into five groups and addeddifferent stimuli separately. Optical density (OD) were assayed with enzyme labelling instrument bycolourmetry and inhibition rats were counted. Results: Proliferation of huMsC cultured in vitrowere significantly stimulated by IL-1, whereas significantly inhibited by SSN. Inhibition rat isrelative to dosage. Confusion: proliferation of huMsC stimulated by IL-1 were significantly inhibited bycontainig SSN rat serum. It was indicated that huMsC is the important target cells for treatment ofSSN.