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荧光标记复合扩增STR检测技术在法医学中的应用 被引量:4

FORENSIC APPLICATION OF FLUORESECNT STR MULTIPLEX AMPLIFYING SYSTEM WITH 8 STR LOCI AND AMELOGENIN
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摘要 本研究用荧光标记8个STR位点及性别位点,于同一试管同步进行扩增后,在PE377全自动测序仪于一个泳道内,成功地对各位点进行基因型分离检测.将此方法应用于30多起案件检验,均取得满意结果. A fluorescent STR system contains D16S539, D7S820, D13S317, D5S818, CSF1PO, TPOX, TH01, VWA STR loci and Amelogenin locus was amplified in a tube and moved by electrophoresis in a gel lane. The genotype of all 9 loci was successfully analyzed and 30 cases were solved with this method. The system is a sensitive, quick and have high value in routine casework.
出处 《刑事技术》 北大核心 1999年第3期13-15,共3页 Forensic Science and Technology
关键词 复合扩增 STR分型 荧光标记 STR typing Co - amplification Fluorescein labelling
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  • 1赵兴春,叶健.法庭DNA技术的发展及展望[J].刑事技术,1998,23(5):41-44. 被引量:5
  • 2(美)CW迪芬巴赫,(美)GS德弗克斯勒,PCR技术实验指南[M].译者:种康,瞿礼嘉.北京:化学工业出版社:2006.
  • 3(美)J.萨姆布鲁克.分子克隆实验指南[M].北京:科学出版社:2008.
  • 4Dennis Y Wang, Chien-Wei Chang, Nicola J Oldroyd, etc. Direct amplification of STRs from blood or buccal cell samples[J]. Forensic Science International:Genetics Supplement Series. 2009,2(1): 113-114.
  • 5Dennis Y. Wang, Chien-Wei Chang, Lori K. Hen nessy. Rapid STR analysis of single source DNA sam ples in 2 h[J]. Forensic Science International: Genetics Supplement Series. 2009,2(1): 115-116.
  • 6Adrian Linacre, Vera Pekarek, Yuvane_swari Cbandramoulee Swaran,etc. Generation of DNA profiles from fabrics without DNA extraction[J]. Forensic Science International: Genetics Supplement Series. 2010, 4 (2): 137-141.
  • 7Levadokou E N,Freeman D A,Budzynski M J,et al.Allele frequencies for fourteen STR loci of the PowerPlexTM 1.1 and 2.1 Multiplex Systems and Penta D locus in Caucasians,African -Americans,Hispanics and other populations of the United States of America and Brazil[J].J Forensic Sci,2001,46:736 -761.
  • 8Lins A M,Micka K A,Sprecher C J,et al.Development and population study of an eight-locus short tandem repeat (STR) multiplex system[J].J Forensic Sci,1998,43:1168-1180.
  • 9Smith J R,Carpten J D,Brownstein M J,et al.Approach to genotyping errors caused by nontemplated nucleotideaddition by TaqDNA polymerase[J].Genome Res,1995,5:312-317.
  • 10Brinkmann B,Moller A,Wiegand P,et al.Structure of new mutations in 2 STR systems[J].Int J Leg Med,1995,107:201 -203.

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