骨巨细胞瘤M-CSF、IL-1和TNF-α免疫组织化学研究

AN IMMUNOHISTOCHEMICAL STUDY OF M CSF、IL 1 TNF α IN GIANT CELL TUMOR OF BONE

本实验应用免疫组织化学方法，检测巨噬细胞相关抗原、巨噬细胞集落刺激因子（Ｍ┐ＣＳＦ）、白细胞介素┐１（ＩＬ┐１）和肿瘤坏死因子┐α（ＴＮＦ┐α）在骨巨细胞瘤（ＧＣＴ）中的表达，结果表明，在ＧＣＴ中，Ｍ┐ＣＳＦ主要由纤维母细胞样基质细胞（ＦＣ）分泌；巨噬细胞样基质细胞（ＨＣ）是单核┐巨噬细胞系早期的未成熟细胞，主要分泌ＩＬ┐１和ＴＮＦ┐α，少数多核巨细胞（ＭＧＣ）也可产生ＴＮＦ┐α。本文提示，ＧＣＴ中的三种主要细胞能分泌Ｍ┐ＣＳＦ、ＩＬ┐１和ＴＮＦ┐α细胞因子，通过自分泌或旁分泌作用相互影响，共同促进ＧＣＴ的发生。

In this study, immunohistochemical method was used to detect the expression of macrophage associaeted antigens, macrophage colony stimulating factor (M CSF), interleukin 1 (IL 1) and tumor necrosis factor α (TNF α) in giant cell tumor of bone (GCT). The results showed that M CSF was mainly secreted by fibroblast like stromal cells (FC) in GCT. Histocyte like stromal cells (HC) were immature cells in the early stages of monocyte macrophage lineage, and they had the ability to secret IL 1 and TNF α. Some of the multinucleated giant cells (MGC) could also produce TNF α. These facts indicated that the three major components of FC, HC and MGC in GCT could act each other by autocrining or paracrining of M CSF, IL 1 and TNF α to promote generation, growth and invading biological behaviors of GCT.