摘要
本文研究了大鼠海马内微量注射甲硫－脑啡肽（Ｍ－ＥＮＫ）对海马细胞的白细胞介素－６（Ｉｎｔｅｒｌｅｕｋｉｎ－６，ＩＬ－６）基因表达的影响。大鼠双侧海马内微量注射细菌内毒素脂多糖各１μｌ（ＬＰＳ，浓度：５０ｎｇ／ｍｌ），于９０ｍｉｎ后用原位杂交技术检测到海马结构ＩＬ－６的基因表达，以齿状回颗粒细胞层显著。当海马内预先注射Ｍ－ＥＮＫ（每侧１μｌ，浓度：１０μｇ／μｌ），３０ｍｉｎ后再给予脂多糖则未见ＩＬ－６基因表达。结果表明Ｍ－ＥＮＫ及ＬＰＳ可影响脑内ＩＬ－６的基因表达，在中枢调节机体免疫功能中，ＩＬ－６可能具有重要作用。
The effect of intrahippocampal
microinjection of enkephalin on interleukin 6 (IL 6) gene expression in hippocampus neurons
of rat was studied using in situ hybridization method. The IL 6 gene expression was detected
in dentate gyrus of hippocampus 90 min after microinjecting 1 μl of lipopolysaccharide (LPS,
50 ng/μl) into bilateral hippocampus. The expression of IL t mRNA induced by LPS was
located in granule cells of the dentate gyrus. The LPS induced IL 6 gene expression in granule
cell layer was inhibited by pre injecting 1 μl of Met enkephalin (M ENK, 10 μg/μl) into
bilateral hippocampus. The results suggest that M ENK and LPS can influence IL 6 gene
expression in the hippocampus and IL 6 might play an important role in central
neuroimmunomodulation.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第2期8-12,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金
