摘要
以改良Ａｌｅｎ氏法造成Ｗｉｓｔａｒ大鼠不完全性脊髓损伤，采用神经学功能评分法评定大鼠运动功能，应用定量酶细胞化学方法观察脊髓前角运动神经元内乙酰胆碱酯酶（ＡＣｈＥ）和酸性磷酸酶（ＡｃＰ）活性变化。结果显示：１．脊髓损伤后大鼠运动功能障碍，随后逐渐恢复。２．前角运动神经元内ＡＣｈＥ活性减弱、ＡｃＰ活性增强；随后酶活性呈逐渐恢复，四周时ＡＣｈＥ活性基本恢复正常。结果说明：大鼠脊髓不完全性损伤后运动功能变化与前角运动神经元的功能状态具有较强的相关性；前角运动神经元在不完全性脊髓损伤运动功能恢复中起重要作用。
Incomplete spinal cord injury in the Wistar rats was produced using the
modified Allen method. Rat motor function was evaluated by means of neurologic scoring. The
acetylcholinesterase (AChE) activity and acid phosphatase activity in anterior horn cells were
observed by using the quantitative enzyme cytochemistry. The results showed: 1. There
appeared rat motor functional deficits after injury, followed by gradual recovery. 2. AChE activity
in anterior horn cells dcreased and AcP activity increased; enzyme activity then gradually
recovered and AChE activity restored basically to normal level four weeks after injury. The
results indicate there is a strong correlation between the rat motor function and the functional
state of anterior horn cells. The anterior horn cells play an important role in the motor functional
recovery following incomplete spinal cord injury.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第2期57-60,共4页
Chinese Journal of Histochemistry and Cytochemistry
基金
辽宁省科学技术委员会基金
关键词
脊髓损伤
乙酰胆碱酯酶
酸性磷酸酶
运动功能
Spinal cord injury
\
Acetycholinesterase
\ Acid phosphatase
\ Motor function
