地高辛标记探针电镜原位杂交技术探讨

A STUDY ON THE TECHNIQUE OF ELECTRON MICROSCOPIC IN SITU HYBRIDIZATION USING DIG LABELLED PROBE

应用ＬｏｗｉｃｒｙｌＫ４Ｍ低温包埋，Ｄｉｇ标记ｃ－ｆｏｓｃＤＮＡ探针对人肝癌组织进行原位杂交，用金标抗Ｄｉｇ抗体进行检测，银增强放大信号处理。电镜观察表明，免疫胶体金颗粒特异性地标记在肝癌细胞胞浆及核内，呈散在分布，金颗粒大小基本一致，背景清晰。

A study of electron microscopic in situ hybridization was performed on the human hepatocellular carcinoma (HCC) tissue with the aid of Dig labelled c fos cDNA probe. The tumor tissue was embedded under -20℃ in Lowicryl K4M and the hybridization effects were detected by use of gold labelled anti Dig antibody. The hybridization signal was enhanced by silver treatment. The electron microscopic observation showed that the immunocolloid gold particles were specifically localized in the cytoplasm and the nuclei of the HCC cells. The gold particles revealed an uniform shape and size and the background of the sections was clean and clear. The advantage of this method would be discussed.