癫痫大鼠大脑皮质及海马神经元NOS的变化

THE CHANGES OF NOS IN CEREBRAL CORTEX AND HIPPOCAMPUS OF EPILEPTIC RAT

给大白鼠侧脑室注射马桑内酯（Ｃｏｒｉａｒｉａ Ｌａｃｔｏｎｅ， ＣＬ）（１７５×１０－ ２ｍ ｏｌ／Ｌ２μｌ）后可诱发癫痫，用ＮＡＤＰＨｄ 组织化学方法观察大脑皮质及海马ＮＯＳ阳性神经元的变化， 结果： 大脑皮质ＮＯＳ阳性神经元数目逐渐增加， 至２ｈ 达高峰， 与生理盐水组相比差异具有非常显著性意义（Ｐ＜ ００１）， 随着ＣＬ作用时间延长ＮＯＳ反应由弱变强；海马区ＮＯＳ阳性神经元２ｈ 时才出现染色明显加深。对体外培养的大脑皮质及海马神经元用ＣＬ （２５×１０－ ５ｍ ｏｌ／Ｌ） 作用１／２ｈ、１ｈ、２ｈ、４ｈ 后ＮＯＳ阳性神经元均未见明显增加。

Epilepsy was kindled by injecting 2μl of Coriaria Lactone (CL, 1 75×10 -2 mol/L) into the lateral ventricle of rats, then the Nitric Oxide Synthase (NOS) positive neurons in cerebral cortex and hippocampus were detected by NADPH d histochemistry. The number of NOS positive neurons in cortex increased at the beginning of experiment, and reached the peak after 2h. There was significant difference compared with the control group(P<0 01). The intensity of NADPH d stain increased with the extension of time. In hippocampus neurons were stained stronger after 2h challenged with CL. But NOS positive neurons did not increase in vitro.\;