大鼠小肠移植后一氧化氮合酶组织化学观察

HISTOCHEMICAL OBSERVATION OF NITRIC OXIDE SYNTHASE AFTER RAT SMALL BOWEL TRANSPLANTATION

监测大鼠小肠移植后一氧化氮合酶的变化与排斥反应的关系以及肝小肠联合移植与单纯小肠移植后的变化异同。两组移植后分别于１、３、７……天同时取肠壁病理和组织化学标本对比，病理为常规ＨＥ染色观察， 一氧化氮合酶（ＮＯＳ） 组织化学检查按Ｄａｗ ｓｏｎ 方法显示其活性。结果两组肠壁ＮＯＳ的活性随移植后排斥反应加重而降低或消失， 单纯小肠移植比肝小肠联合移植变化明显。大鼠小肠移植后肠壁内ＮＯＳ变化与排斥反应相关， ＮＯ

The aim of this study is to monitor the relationship between NOS and rejection following small bowel transplantation and to compare the difference between liver/small bowel graft and simple small bowel graft. All rats were examined pathologically and histologically in the 1st, 3rd and 7th day after transplantation. The specimen was stained with hematoxylin and eosin for pathological examination. The activity of NOS in enteric nerves was observated histologically using Dawson method. The results suggested that activity of NOS in enteric graft was decreasing or disappearing during heavier rejection. The activity of NOS in simple small bowel transplantation was signficantly decreased than that in liver/small bowel transplantation. The present study showed that the change of enteric NOS was correlated with small bowel allograft rejection reaction. Thus, NOS activity may be used as one of possible monitoring markers of allograft rejection following small bowel transplantation.\;