肝细胞癌p53蛋白过表达的免疫组织化学研究

IMMUNOHISTOCEMICAL STUDY OF p^(53) PROTEIN OVEREXPRESSION IN HEPATOCELLULAR CARCINOMAS

为探讨肿瘤抑制基因ｐ５３ 在肝癌细胞中的变化， 本实验对３８ 例人肝细胞癌组织ｐ５３ 蛋白的表达进行了免疫组织化学检测， 并与其临床和病理观察进行了比较研究。免疫组化检查显示， １５例肝癌组织ｐ５３蛋白免疫染色阳性， 其突变率为３９．５％ （１５／３８）。比较表明， 肝细胞癌ｐ５３ 蛋白的过度表达与病人的年龄、性别和肿瘤大小无关， 而与肝癌的转移和分化程度有关， 在高分化肝癌中其阳性率为９．１％ （１／１１）， 在中分化肝癌为２２．２％ （２／９）， 在低分化肝癌则高达６６．７％ （１２／１８）。因此， ｐ５３

In order to study the p53 gene mutation in hepatocellular carcinoma, the overexpression of p53 protein in 38 cases was detected using immunohistochemical technique, ABC. The overexpression of p53 protein was found in 15 (39 5%) of 38 patients. Statistically, no significant relationship between overexpression of p53 protein and clinical data including age, sex and tumor size was observed. However, the p53 mutation was closely related with tumor metastasis and the degree of differentiation of the tumor. Immunostaining positive p53 protein was found 9 1% (1/11), 22 2% (2/9) and 66 7% (12/18) in highly, moderately and poorly differentiated hepatocellular carcinomas, respectively. It indicates that the presence of overexpression of p53 protein may become one useful prognostic parameter for hepatocellular carcinomas.\;