摘要
为探讨肿瘤抑制基因p53 在肝癌细胞中的变化, 本实验对38 例人肝细胞癌组织p53 蛋白的表达进行了免疫组织化学检测, 并与其临床和病理观察进行了比较研究。免疫组化检查显示, 15例肝癌组织p53蛋白免疫染色阳性, 其突变率为39.5% (15/38)。比较表明, 肝细胞癌p53 蛋白的过度表达与病人的年龄、性别和肿瘤大小无关, 而与肝癌的转移和分化程度有关, 在高分化肝癌中其阳性率为9.1% (1/11), 在中分化肝癌为22.2% (2/9), 在低分化肝癌则高达66.7% (12/18)。因此, p53
In order to study the p53 gene mutation in hepatocellular carcinoma, the overexpression of p53 protein in 38 cases was detected using immunohistochemical technique, ABC. The overexpression of p53 protein was found in 15 (39 5%) of 38 patients. Statistically, no significant relationship between overexpression of p53 protein and clinical data including age, sex and tumor size was observed. However, the p53 mutation was closely related with tumor metastasis and the degree of differentiation of the tumor. Immunostaining positive p53 protein was found 9 1% (1/11), 22 2% (2/9) and 66 7% (12/18) in highly, moderately and poorly differentiated hepatocellular carcinomas, respectively. It indicates that the presence of overexpression of p53 protein may become one useful prognostic parameter for hepatocellular carcinomas.\;
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1999年第4期448-451,共4页
Chinese Journal of Histochemistry and Cytochemistry