重组葡激酶的基因克隆与序列测定

Cloning and sequencing of recombinant staphylokinase

从烧伤病房病人烧伤感染创面分离到 9株金黄色葡萄球菌 ,经纤溶活性测定 ,选纤溶活性最高的作为 PCR扩增模板。将扩增出的葡激酶基因片段插入 p UC1 8载体质粒 ,转化大肠杆菌 DH5 α,筛选出 PUC- SAK阳性克隆 ,经 DNA序列测定证实该基因片段为一种新的

Nine Strains of Staphylococcus aureus were isolated from some patients who suffer from bacterial infection in a burn ward of a hospital. After detecting of fibrinolytic activity in all of the strains, select the strain with strong fibrinolytic activity as a template of PCR amplification. PCR products insert in cloning vector pUC18 and transformed into E.coli DH5α. Screen the positive cloning which contains the cDNA of staphylokinase, DNA sequencing demonstrated that it were novel.