不同群系小鼠胚胎玻璃化冷冻保存技术的研究

Cryopreservation by vitrification of embryos from different colonise of mice

利用 EFS40 ,二步法对近交系 C5 7BL/6、DBA/2和远交群 ICR小鼠囊胚玻璃化冷冻保存 ,并对冷冻后胚胎体内、外发育效果进行比较。结果表明 ,相同条件下鲜胚经培养 ,近交系 C5 7BL /6小鼠的囊胚发育率 ( 93% )与 ICR( 1 0 0 % )相比差异不显著 ( P>0 .0 5 ) ;而两近交系的囊胚孵化率明显低于 ICR( P<0 .0 1 )。 C5 7BL/6、DBA/2小鼠囊胚冷冻后发育率 ( 93% ,96% )和孵化率 ( 5 2 % ,46% )与各自对照组 ( 1 0 0 % ,1 0 0 %和 61 % ,62 % )相比均无显著差异 ( P>0 .0 5 ) ;并且与 ICR冷冻组发育率和孵化率 ( 94% ,5 3% )之间也无显著差异 ( P>0 .0 5 )。两近交系冻胚移植妊娠与各自对照组和 ICR冷冻组比较均无显著差异 ( P>0 .0 5 )。C5 7BL/6胚胎移植产仔率 ( 35 % )与对照组 ( 5 1 % )之间差异显著 ( P<0 .0 1 ) ,而 DBA/2胚胎移植产仔率 ( 4 7% )与对照组和 ICR冷冻组 ( 39% ,5 8% )相比差异不显著 ( P>0 .0 5 )。

Blastocysts from inbred strains C57BL/6, DBA/2, and outbred stock ICR mice were cryopreserved by two step method in vitrification solution of EFS40, and their post thawed developmental potential in vivo and in vitro were compared among the colonies. The result showed when the morulae were cultured without exposed to cryopreservation, there was significant difference in the rates of hatched blastocysts between C57BL/6, DBA/2 and ICR mice (P<0 01), but not in survival percentage of morulae development between C57BL/6 (93%), and ICR (100%). After the embryos were cryopreserved by vitrification, the survival percentages, the rates of hatched blastocysts and pregnant rate of receipts received cryopreserved embryos from C57BL/6, DBA/2 were not lower than those of control (embryos uncryopreserved) and ICR, respectively. There was no marked differences in giving birth rates among the transferred receipts received embryos from different colonies of mice.