混合DNA样品池扩增法及其应用

Pooled DNA amplification and its application

将个体 DNA提取出来后 ,按一定方式进行混合 ,构成混合 DNA样品池。这种混合 DNA样品可用于病因未明的遗传性及遗传易感性疾病的研究。在研究常染色体隐性遗传性耳聋致病基因时 ,发现与染色体 9q的 D9S92 2和 D9S30 1位点有相关性。此方法比通常的连锁分析法省时省力。在肿瘤相关基因或责任基因的研究、法医学的个体认定、基因突变的检测等方面均显示出实用性 。

After individual genomic DNA was extracted, it would be pooled to formate DNA pooling in some ways. The pooled DNA sample can be used as a study and examination tool for loci of genetic disease and susceptibility disease gene. While linking analysis an autosomal recessive Nonsyndrome Hearing Loss locus, amplification of DNA pooling was revealed for linkage with makers D9S922 and D9S301 on chromosome 9q. This method was labour saving and cost saving. It had been applied successfully in study of relative gene in different tumors, forensic identification of individuals, detection of gene mutation etc. It was a valuable method.