摘要
将测序后的葡激酶重组质粒ＰＵＣ－ＳＡＫ经酶切后，组装于表达载体ｐＢＶ２２０，构建成ｐＢＶ－ＳＡＫ表达质粒，转化大肠杆菌。重组葡激酶表达水平达６０％～７０％，相对分子质量为 １５ ５００，主要以可溶状态存在于细胞中。生物活性测定证实，重组葡激酶具有很强的纤溶活性。
The recombinant expression plasmid pBV-SAK was constructed by inserting the cDNA fragment of staphylokinase into pBV220 and transformed into E. coli DH5α cell. Induction of transfected E. coli DH5α cell in a bacterial fermentor produced intracellular recombinant staphy- lokinase representing 60%-70% of total cell protein and in soluble form. Staphylokinase activity was measured by using the fibrin plate method.
出处
《生物技术通讯》
CAS
1999年第4期266-269,共4页
Letters in Biotechnology
关键词
葡激酶
基因表达
生物活性
recombinant staphylokinase
gene expression
biological activities
