苏木不同提取物对小鼠胸腺和T细胞增殖功能的影响

Effect of different caesalpinia sappan extract on mice thymus and proliferating function of T-cells

目的:观察苏木不同提取物对小鼠胸腺组织、外周T细胞增殖和产生INF-γ的影响。方法:制备苏木不同提取物分别给予灌胃小鼠,7d后取小鼠胸腺称重计算胸腺指数;采用MTT法测定小鼠脾T细胞的增殖能力;用双抗体夹心ELISA法测定T细胞培养上清中产生INF-γ的含量。结果:苏木不同提取物不仅对胸腺重量具有明显的抑制作用,而且还对T细胞的增殖和产生INF-γ的能力具有明显的抑制作用。其中苏木醇提取物和正丁醇提取物对T细胞的抑制作用较强。

Objective： To observe the effect of different caesalpinia sappan extract on the Mice thymus tissue, peripheral T-cell proliferation and the production of INF-γ. Methods： To make preparation of different caesalpinia sappan extract and give them to the mice, weighing the mice thymus and calculating the thymus index 7 days later. The MTT method was used to measure the proliferation ability of T-cells of the mouse spleen. A double antibody sandwich ELISA was used to detect INF-γ content in T-cells culture supernatant. Results： The different caesalpinia sappan extract not only have clear inhibitory effect on the weight of thymus but also have inhibitory effect on the proliferation of T-cells and the production of INF-γ. The inhibitory effect of caesalpinia sappan ethanol extract and n-butanol extract on the T-cells is much stronger.