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The gene diagnosis and mutation survey of autosomal dominant polycystic kidney disease Ⅰ

The gene diagnosis and mutation survey of autosomal dominant polycystic kidney disease Ⅰ
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摘要 Objective: To explore gene diagnosis of autosomal dominant polycystic kidney disease (ADPKD Ⅰ ),and to look for the typical mutation in order to improve the gene diagnosis. Metbods: southern blot and PCR wasused to observe the mutation condition of 3' end single copy region of ADPKD Ⅰ gene ; Amplifying and analyzingthe microsatellite SM7 by PCR. Results: ①After the probe AH4 was hybridized with 16 patients' genomic DNAby Southern blot, the common 15 kb fragments were found in every one; ②For 27 patients, 5. 72 kb genomicDNA, which is between the probe AH4 and JH14, was amplified by PCR, and no 5. 5 kb genomic DNA deletionwas found in this region; SM7 was amplified in lO9 health persons, its PIC was 0.76, and was closely linked towith ADPKD Ⅰ gene in three patient families. Conclusion: In Han nation, ①No large genomic DNA segmentdeletion could be found frequently in ADPKD Ⅰ gene 3' end single copy region; ②The PIC of SM7 is high, it canbe used to make rapid gene diagnosis in about 70% ~80% ADPKD Ⅰ families.
出处 《Journal of Medical Colleges of PLA(China)》 CAS 1999年第4期299-303,共5页 中国人民解放军军医大学学报(英文版)
关键词 ADPKD SM7 GENE DIAGNOSIS ADPKD Ⅰ SM7 gene diagnosis
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参考文献7

  • 1Saris JJ,Breuning MH,Daunerse HG et al.Rapid detection ofpolymorphism near gene for adult polycystic kidney disease. The Lancet . 1990
  • 2Harris PC,Thomas S,Ratcliffe PJ et al.Rapid genetic analysis offamilies with polycystic kidney disease 1 by means of a microsatellite marker. The Lancet . 1991
  • 3Readers ST,Breuning MH,Davies AE et al.A highly polymorphic DNA marker linked to adult polycystic kidney disease onchromosome 16. Nature . 1985
  • 4Wang Zhireng,Tang Deyun.Liang chaidong et al.A survey report of 11807 persons on occurrence rate of simple renal cyst andpolycystic kidney in Chen De area. Clin Urosurg . 1993
  • 5The European Polycystic Kidney Disease Consortium.The polycystic kidney disease 1 gene encodes a 14 kb transcript and lieswithin a duplicated region on chromosome 16. Cell . 1994
  • 6Iglesias D,Fraga AR,Arrizurieta E et al.Atrial myxoma in awoman with autosomal dominant polycystic kidney disease type2. American Journal of Kidney Diseases . 1997
  • 7Schneider MC,Zhang F,Geng L et al.Identification of the autosomal dominant polycystic kidney disease gene,PKD1. The American Journal of Human Genetics . 1994

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