我国不同地区白木香核糖体DNA内转录间隔区碱基序列分析

Ribosomal DNA ITS sequence analysis of Aquilaria sinensis from different geographical origin in China

目的:研究我国不同地区白木香核糖体DNA(rDNA)内转录间隔区(ITS)碱基序列的差异。方法:运用PCR法对白木香的ITS(包括ITS1,5.8S,ITS2)序列扩增后直接测序,用软件CLUSTALX和MEGA分析测序结果。结果:白木香ITS区总长度为680bp,其中ITS1为246bp,5.8S为163bp,ITS2为271bp。ITS序列在白木香种内的变异很小,只有6个变异位点,种内遗传距离为0.0%-1.1%。结论:ITS序列的差异为鉴别不同产区白木香及其近缘种提供了依据。

Objective： To study rDNA ITS （internal transcribed spacers） sequences variation from di erent population of Aquilaria sinensis in main habitat of China. Methods： The rDNA ITS regions of various A. sinensis were ampli ed by PCR method and sequenced, and they were analyzed by means of the software of CLUSTAL and MEGA. Results： The sequences of rDNA ITS region of A. sinensis were reported for the rst time, and the sequences of ITS region were 680bp （ITS1 246bp, 5.8S 163bp, ITS2 271bp）. There were 6 variable sites among populations and the genetic distance were 0.0% to 1.1%,which indicated the intraspecific genetic variation was low of A. sinensis. Conclusion： The variation of rDNA ITS sequences can be used to authenticate A.sinensis from di erent geographical regions and their adulterants.