胆囊结石患者胆汁需氧菌和厌氧菌脱氧核糖核酸检测及意义

Measurement and significance on deoxyribonucleic acid of aerobic and anaerobic bacteria in bile from patients with gallbladder gallstones

目的:为探索提高胆囊结石患者胆汁细菌检出阳性率,从分子生物学水平研究胆囊结石胆汁细菌脱氧核糖核酸(DNA)。方法:采用聚合酶链式反应(PCR)对胆汁中5种需氧菌和4种厌氧菌DNA进行检测。结果:检测70例其中需氧菌DNA存在56例(80％),厌氧菌DNA存在23例(32.9％)。两类细菌DNA共同存在为20例(28.6％)。从21例胆汁中检测出幽门螺杆菌DNA。结论:胆结石刖汁中存在多种细菌DNA。PCR可提高细菌检出阳性率。

Objective: To impreve positive rate of baritecta detetion in bile from patients with gallbladder gallstone by means of measuremeut of deoxyribonucleic acid (DNA) of bacteria using molecular biolgi-cal procedures. Methods: DNA of five aerobic and four anaerobic bacteria in bile were measured with poly-merase chain reaction (PCR). Results: 70 patients were studied, there were DNA of aerobic and anaerobic in 56 patients (80%) and 23 patients (32. 9%), respectively. There were DNA of Both bacteria in 20 patients (28. 6%). DNA of Helicobacter Pylori was measured in 21 patients' bile. Conclusion: There were multiple bacteria DNA in the bile from patients with gallbladder gallstones the positive rate of bacteria detection in bile may be improved by PCR.