茶树种质资源抗茶云纹叶枯病鉴定

Evaluation of Resistance of Tea Germplasm Resources to Tea Brown Blight(Colletotrichum camelliae Massee)

茶树种质资源抗茶云纹叶枯病鉴定,进行了田间自然发病调查、苗圃茶苗和室内离体茶枝人为接种试验.室内离体接种诱发的病叶还进行了病原菌的再分离.室内离体接种的病情指数与苗圃中接种的病情指数及自然发病调查的病情指数的相关系数分别为r= 0.6993、r= 0.4036,可见离体接种试验结果与苗圃试验和自然发病调查结果具较好吻合性.离体茶枝接种诱发的病叶经分离培养均为该病的分生孢子.确定离体茶枝接种为该病抗性的鉴定方法,拟定抗性等级划分标准,筛选出高抗茶资源1份、抗的12份、中抗的13份.大叶种的抗病性差于中叶种的茶资源.

The resistance of tea germplasm resources to tea brown blight was evaluated by field investiga-tion, nursery inoculation and detached shoot inoculation. The results showed that the disease index of inocu-lated detached shoots was significantly correlated to that of shoots inoculated in the nursery and that natural-ly occurring in the of field (r = 0. 6993**, r = 0. 4036*, P< 0. 01 and P< 0. 05 respectively). The conid-iospores of Colletotrichum camelliae Massee were isolated from inoculated, diseased leaves. Detached shootinoculation is recommended as the prefersed method for resistance evaluation. A resistance grading system isoutlined. One highly resistant, 12 resistant and 13 medium-resistant germplasms were screened out. Large-leaf germplasms were found to have weaker resistance than medium-and small-leaf germplasms.