摘要
Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.
Objective To investigate the consistent inhibitory effect olperitoneal dialysate on the capacity ofperitoneal macrophage release of TNF- α and the inhibition associated with different glucose concentration invivo. Methods We compared the peritoneal macrophage activity obtained from the irrigation solution of theuremia patients (n=2) immediately after inserting the catheter and with those from the long - dwell time dialysate(≥10h) of peritoneal dialysis patients (n= 7). The capacity of peritoneal macrophage to release TNF - x was assayedby L929 cytotoxicity. At the same time, we compared TNF- 7 activity among different glucose concentration (1.5%,2.5% and 4.25% dextrose) of dialysate. Results The levels of TNF- a in CAPD and control group were 170 and517 pg/106 respectively (P<0.01). The patients who were using dialysate containing higher glucose concentrationhad lower TNF- x level (P<0.05, P<0.01). Conclusion It is suggested that the inhibitory effect of peritonealdialysate on peritoneal macrophages might continue at least 10h in vivo. The higher glucose concentration ofdialysate may have worse effect on peritoneal macrophage activity.
