周围神经缺血再灌注损伤及药物保护研究

Study of reperfusion injury of ischemic peripheral nerve and drug protection

目的:观察周围神经缺血再灌注损伤和不同药物保护时形态学及形态计量的变化.方法:采用光镜和电镜下观察神经形态结构的改变,应用计算机图像分析系统进行灰度对比分析.结果:周围神经缺血再灌注时,形态学损伤程度与缺血再灌注时间有关,缺血时间越长,组织形态学变化越明显,再灌注后改变进一步加重;甘露醇、氯丙嗪和当归可减轻缺血性损伤的改变.结论:甘露醇、氯丙嗪和当归对周围神经缺血再灌注损伤具有保护作用.

ve: To observe the reperfusion injury of ischemic peripheral nerve and the morphologic changes when various drugs are used for protection. Method: Morphologic changes were observed under light and electron microscopes and computer imaging analysis system was used to analyse the degrees of grey value under the lihgt microscope. Result: Morphologic damages were related to the time of ischemia and reperfusion. The longer the ischemic duration , the more obvious the morphologic changes, and even more obvious after reperfusion. The extents of tissue damage were inhibited after administration of mannitol, Danggui or chlorpoma-zine. Conclusion: Mannitol, Danggui and chlorpromazine prove to be preventive against reperfusion injury of peripheral nerve.