PCR克隆EB病毒潜伏膜蛋白-1基因

Cloning of the Latent Membrane Protein-1 Gene of Epstein-Barr Virus

目的：构建ＥＢ病毒潜伏膜蛋白－１重组表达质粒。方法：ＰＣＲ克隆技术，即用ＰＣＲ方法从Ｂ９５－８细胞中钓出ＥＢ病毒ＬＭＰ１基因ＤＮＡ序列，然后定向克隆到真核表达质粒ｐｃＤＮＡ３．１中，以此构建成ｐｃＤＮＡ３．１－ＬＭＰ重组质粒。结果：经酶切鉴定，确定已获得重组质粒ｐｃＤＮＡ３．１ＬＭＰ１。结论：在真核表达质粒中已成功地克隆了ＥＢ病毒ＬＭＰ１基因。

Objective: To construct a recombinant plasmid with expresion of latent membrance protein-1(LMP- 1) of Epstein-Barr Virus(EBV). Methods:With PCR cloning technique, the full length of EBV LMP-1 gene from B95 - 8 Cells was amplified and then cloned directionally into vector plasmid pc DNA3. 1, thus the recombinant plasmid pc DNA3. 1-LMP-1 was constructed. Results: By restriction enzyme digestion, it was confirmed that the recombinant plasmid pcDNA3.1-LMP-l had been successfully acquired.