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小麦抗白粉病基因Pm13的标记辅助选择 被引量：3

Marker-Assisted Selection of Pm13 Gene Conferring Resistance to Powdery Mildew in Wheat
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摘要 选用"滚动式加代回交转育"抗白粉病育种圃中含有 Pm13抗白粉病基因的18个杂交组合,结合温室抗病性鉴定,利用与 Pm13基因紧密连锁的 SCAR 标记对 Pm13抗白粉病基因进行了分子鉴定。试验结果表明,Pm13基因在不同的遗传背景下对北京地区优势白粉病菌15号小种表现抵抗。对18个组合处于不同遗传世代的68个抗感单株的分子标记鉴定结果与抗病性鉴定结果具有高度的一致性,抗病植株中均可以扩增出575bp 的 DNA 片段,而感病植株没有扩增产物。利用这一 SCAR 标记对 Pm13基因进行检测具有快速、准确、可靠的优点,可应用于小麦抗白粉病育种中的标记辅助选择和抗白粉病基因的积累。 Tightly linked SCAR marker was used to detect powdery mildew resistance gene Pm13 originated from Aegilops longissimum in different generations of 18 different crosses of a wheat breeding program.The results indicated that a 575 bp DNA fragment was linked to the resistance gene and the molecular identification was well in agreement with the results of inoculation-powdery mildew resistance tests.The highly reproducible SCAR marker make it very useful in marker-assisted selection(MAS)and pyramiding Pm13 gene with other Pm genes in wheat breeding.
作者 刘志勇 孙其信 倪中福 杨作民 杨爱东 唐伯让
机构地区 中国农业大学植物遗传育种系
出处 《中国农业大学学报》 CAS CSCD 北大核心 1998年第S4期12-18,共7页 Journal of China Agricultural University
基金 本研究为国家自然科学基金 39670508
关键词 标记辅助选择 Pm13 SCAR 白粉病 小麦 marker assisted selection(MAS) powdery mildew Pm13 SCAR wheat
分类号 S512.1 [农业科学—作物学]
  • 相关文献

参考文献14

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二级参考文献5

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共引文献90

同被引文献38

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引证文献3

二级引证文献28

中国农业大学学报
1998年 第S4期

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