GA_4间接酶联免疫吸附测定方法的建立

Indirect Enzyme-linked Immunosorbent Assay for Determination of Free Gibberellin A_4

利用 GA_4-17-BSA 作为免疫抗原,GA_4-16-OA 作为包被抗原,建立了检测自由态 GA_4的间接ELISA。检测极限为4.1ng/mL,抗血清与 GA_7,GA_3,GA_4-ME 的交叉反应率分别为68.51%,9.10%。1.90%,与 IAA,ABA,ZR 交叉反应率极小。

Gibberellin A_4 was coupled to bovine serum albumin(BSA)via C-17 as antigen (GA_4-17-BSA)in the preparation of antiserum.Antiserum to GA_4-17-BSA conjugate showed high crossreactivity with GA_7 and low cross-reactivities with GA_3,GA_4-ME,IAA, ABA and ZR.Indirect enzyme-linked Immunosorbent Assay(ELISA)was set up using GA_4-16-OA as coating antigen which has a different chemical linkage bridge to the im- munogenic conjugate.The range for measurements of GA_4 extended from 1000 ng/mL to 4.1 ng/mL.