雏鸡感染巨型艾美耳球虫后细胞免疫的研究

Studies on the Cellular Immune Responses of Chickens to Eimeria maxima Infection

通过用地塞米松处理鸡和用特异性抗原作刺激因子的微量全血淋巴细胞转化试验(LPA),研究了雏鸡抗 Eimeria maxima 再感染中细胞免疫现象。结果表明,地塞米松处理可损伤鸡抗 E.maxima 再感染的免疫力.这提示了 CD8+T 细胞、γIFN 及 IL-2等在抗 E.maxima 卵囊抗原刺激因子的微量全血 LPA结果表明,其刺激指数值与鸡抗 E.maxima 再感染抵抗力有关。此反应可用于体外监测鸡体抗 E.maxima再感染的抵抗力。

The cellular immune responses of chickens to Eimeria maxima reinfection were examined by treating immune chickens with dexamethasone(DEX)and conducting whole blood lymphocyte proliferation assays(LPA)using specific antigens.After challenged with E.maxima,the immunized chickens treated with DEX shed some oocysts while oth- er immnized chickens not treated with DEX shed no oocysts.The results showed that DEX impaired the complete immunity of the immunized chickens to E.maxima reinfection, which suggested that T lymphocytes bearing CD8 antigen gamma-interferon(IFN-γ)and interleukin-2(IL-2)etc.Might play a role in the host defense against E.maxima reinfec- tion.The results of the LPA using E.maxima oocysts antigens as stimulants revealed that the stimulation indexes(SI)were associated with the protective immunity of chickens to E.maxima reinfection.Therefore the LPA could be used to assess in vitro the protec- tive immunity of chickens to E.maxima reinfection.