SPDP蛋白偶联技术在抗原构建中的应用

The Method for Proteins Conjugation by SPDP and Its Application in the Preparation of a Complex Antigen

为了制备多功能复合避孕疫苗，利用异型双功能试剂N-琥珀酰胺-3-（2-吡啶二硫）-丙酸酯（SPDP）对复合抗原的构建进行了试验。SDS聚丙烯酰胺凝胶电泳（SDS—PAGE）结果表明抗原的偶联是成功的。葡萄球菌A蛋白（SPA）和牛血清白蛋白（BSA）被偶联到同一载体破伤风类毒素（TT）上，复合抗原的平均表观相对分子质量为3.4×105。该法有可能用于制备多功能复合抗原。

The present study was carried out to combine a complex antigen by a heterbifunctional reagent, N - succinimidyl - 3 - (2 - pyridyldithio) propionate (SPDP). The results of SDS - PAGE showed that Streptococos Protein A(SPA) and bovine serum albumin (BSA) were conjugated with one tetanus toxoid (TT) carrier. The apparent average molecular weight of the complex antigen is 3.4 × 105.These data implicated that conjunction of antigens was successful and this conjugation techniqUe would be useful for the preparation of multifunctional complex antigens.