摘要
为了制备多功能复合避孕疫苗,利用异型双功能试剂N-琥珀酰胺-3-(2-吡啶二硫)-丙酸酯(SPDP)对复合抗原的构建进行了试验。SDS聚丙烯酰胺凝胶电泳(SDS—PAGE)结果表明抗原的偶联是成功的。葡萄球菌A蛋白(SPA)和牛血清白蛋白(BSA)被偶联到同一载体破伤风类毒素(TT)上,复合抗原的平均表观相对分子质量为3.4×105。该法有可能用于制备多功能复合抗原。
The present study was carried out to combine a complex antigen by a heterbifunctional reagent, N - succinimidyl - 3 - (2 - pyridyldithio) propionate (SPDP). The results of SDS - PAGE showed that Streptococos Protein A(SPA) and bovine serum albumin (BSA) were conjugated with one tetanus toxoid (TT) carrier. The apparent average molecular weight of the complex antigen is 3.4 × 105.These data implicated that conjunction of antigens was successful and this conjugation techniqUe would be useful for the preparation of multifunctional complex antigens.
出处
《暨南大学学报(自然科学与医学版)》
CAS
CSCD
1998年第S1期36-40,共5页
Journal of Jinan University(Natural Science & Medicine Edition)
基金
广东省计生委资助