恒河猴与人GDNF基因的直接全血PCR克隆及序列分析

The Gene Cloning of Macaca and Human GDNF by Direct PCR from Whole Blood and Sequence Analysis

利用微量全血直接进行ＰＣＲ扩增，克隆到了猴与人的ＧＤＮＦ基因，经全序列分析证实人ＧＤＮＦ （ｈＧＤＮＦ）基因全部正确，猴 ＧＤＮＦ（ＲｈＧＤＮＦ ＧｅｎＢａｎｋ ＡＦ１０６６７８）基因与人 ＧＤＮＦ基因高度同源，在 ＤＮＡ的 成熟蛋白编码区中二者有７个核苷酸不同，而蛋白中仅有两个氨基酸差异．该研究为ＧＤＮＦ的重组表达及进 一步研究ＧＤＮＦ蛋白的结构与生物学功能打下基础．简要探讨了全血量对ＰＣＲ扩增结果的影响，并对脊椎动 物进化中ＧＤＮＦ的保守性作了比较．

By direct PCR from whole blood, the RhGDNF gene and hGDNF gene were cloned and sequenced. We investigated the best blood volume for PCR amplification. The sequence data of chinese hGDNF gene showed no polymorphism among different races. The sequence data of RhGCNF gene showed seven bases' difference from hGDNF gene, but only two amino acids were different.