烧伤创面渗液IL-12的变化

The change of interleukin-12 in burn wound exudate

目的：观察烧伤创面渗液中白细胞介素１２（ＩＬ－１２）与创面愈合的关系。方法：在换药时采用海绵吸取创面渗液，用免疫酶联方法定量检测其中ＩＬ－１２的含量。结果：在伤后初期的创面渗液中，ＩＬ－１２含量为（９５．３３±３９．７７）ｐｇ／ｍｌ，与正常血清的ＩＬ－１２含量（８１．３３±５５．０２）ｐｇ／ｍｌ比较接近。烧伤２～６ｄ组和７～１１ｄ组ＩＬ－１２含量明显升高，分别为（２２８．３４±１０１．４３）与（２６４．８７±１２７．４２）ｐｇ／ｍｌ。１２ｄ以后进一步增高，１２～１６，１７～２１，＞２１ｄ组分别为（１４３１．０５±１２０６．３５），（１６４０．５８±１１７８．７８），（１９１５．７５±１３２９．６２）ｐｇ／ｍｌ，一直维持在较高的水平。结论：在不同时期创面渗液中ＩＬ－１２含量有所不同，中晚期创面渗液中的含量显著高于早期创面及正常血清中的含量。

Objective: To measure the concentration of interleukin-12 (IL-12) in exudate at different stages of deep burn wound, and to observe their relationship with wound healing. Methods: The exudate of wounds were extracted during dressing change, and the concentrations of IL-12 were measured by ELISA. Results: The concentration of IL-12 was (95.33±39.77) pg/ml in early stage burn wound exudate, which was very closed to (81.33±55.02) pg/ml in normal serum. The concentrations of IL12 in both 2-6 and 7-11 d group rose obviously and were (228.34±101.43) and (264.87±127.42) pg/ml, respectively. Twelve days after burn, they were more higher and kept a higher level all the time, which in 12-16,17-21,>21 d groups were (1 431.05±1 206.35), (1 640.58±1 178.78) and (1 915.75±1 329.62) pg/ml. Conclusion: The concentrations of IL-12 are different from each other at different stages of burn wound and are higher at metaphase and later stage than at early stage. It suggests that IL-12 is related to controlling of inflammation during wound healing.