α_1酸性糖蛋白对中性多形核粒细胞吞噬和杀菌功能的影响

The effects of α_1acid glycoprotein on the specific oxygenation activity of neutrophils in vivo and in vitro

目的：探讨急性期反应质α１酸性糖蛋白（ＡＧＰ）对中性多形核粒细胞（ＰＭＮｓ）吞噬和杀菌功能的影响。方法：大鼠静脉注射ＡＧＰ，以大鼠游移型和非游移型ＰＭＮｓ在吞噬过程中的化学发光为检测指标，并将经ＡＧＰ调理后的ＰＭＮｓ与酵母聚糖混和进行体外实验检测。结果：ＡＧＰ组ＰＭＮｓ的化学发光值大大强于对照组，ＡＧＰ对ＰＭＮｓ氧化发光的增强作用随剂量的增加而增加，但用ＡＧＰ调理的酵母聚糖组，其ＰＭＮｓ化学发光值明显低于用正常大鼠血清调理的酵母聚糖组。结论：应用ＡＧＰ可以明显增加游移型和非游移型ＰＭＮ的吞噬和杀菌活性。

Objective: To investigate the effect of AGP on the oxygenation activity of polymorphonuclear neutrophils(PMNs) both in vivo and in vitro. Methods: Chemiluminescence(CL) of circulating PMNs (non-migrating PMNs) and PMNs harvested from peritoneal cavity (migrating PMNs) were measured in the presence of zymosan opsonized with the serum of normal rats. Results: PMN-CL was significantly higher in AGP group compared with control group, no matter PMNs were obtained from blood or from peritoneal cavity. In vitro study showed a doserelated increase of PMN-CL after PMNs were incubated with AGP for 1 h. However, in AGPopsonized zymosan group, the PMN-CL was significantly lower than that of serumopsonized zymosan group. Conclusion: It is indicated that administration of AGP can significantly increase the specific oxygenation activity both in migrating and nonmigrating PMNs. This effect of AGP is directly on the PMNs rather than acting as an opsonin.