烧伤及延迟复苏对大鼠肝、肺、肾组织PLA_2活性的影响

The effects of burn and delayed fluid resuscitation on the activities of phospholipase A_2 in liver, lung and kidney of rats

目的：观察烧伤及烧伤后延迟复苏对大鼠肝、肺、肾组织磷脂酶Ａ２（ＰＬＡ２）活性的影响。方法：ＳＤ大鼠给予３０％ＴＢ－ＳＡⅢ度烫伤，按不复苏、早期复苏、延迟复苏分组处理，杀鼠取肝、肺、肾制成组织匀浆，测定ＰＬＡ２活性。结果：烫伤后肝、肺、肾组织ＰＬＡ２活性均显著升高，高峰在４～８ｈ；早期复苏组ＰＬＡ２活性较不复苏组明显降低，但仍高于对照组；延迟复苏组ＰＬＡ２活性在肝、肾组织明显高于不复苏组，在肺组织也有升高，但无显著差别。结论：大鼠烧伤后肝、肺、肾组织ＰＬＡ２活性显著升高，及时有效的液体复苏治疗可以降低ＰＬＡ２活性，延迟复苏则可以增强ＰＬＡ２的活性。

Objective:To investigate the effects of burn and delayed fluid resuscitation on the activities of phospholipase A_2(PLA_2)in liver,lung and kidney of rats. Methods: SD rats were scalded with 30%TBSA Ⅲ°. They were divided into no resuscitation group(B), early resuscitation group(BE) and delayed resuscitation group(BD). Liver,lung and kidney of rats were obtained to measure the activities of PLA_2. Results:The activities of PLA_2 in liver,lung and kidney of rats postburn increased significantly.Its peak was during 4 to 8 h postburn. The activity of PLA_2 in BE group was significantly lower than that in B group, but still higher than that in control group. In liver and kidney,the activity of PLA_2 in BD group was significantly higher than that in B group, while in lung there was only slight increase. Conclusion: Burn can significantly increase the activities of PLA_2 in liver,lung and kidney, which could be decreased by timely and effective fluid resuscitation. However,delayed fluid resuscitation increases the activity of PLA_2, hence aggravating the injury of tissue. 