Immunogenic efficacy of differently produced recombinant vaccines candidates against Pseudomonas aeruginosa infections

Ｐｓｅｕｄｏｍｏｎａｓａｅｒｕｇｉｎｏｓａｉｓａｃｏｍｍｏｎｃａｕｓｅｏｆｉｌｎｅｓｓａｎｄｄｅａｔｈｉｎｉｍｍｕｎｏｃｏｍｐｒｏｍｉｓｅｄｐａｔｉｅｎｔｓ．Ｉｎｐａｒｔｉｃｕｌａｒ，ｔｈｅｒｉｓｋｔｏｔｈｏｓｅｓｕｆｅｒｉｎｇｆｒｏｍｓｅ－ｖｅｒｅ...

Objective: To describe the expression and immunogenic efficacy of differently produced recombinant vaccines candidates against Pseudomonas aeruginosa infections. Methods: The hybrid protein OprF (aa 190342)OprⅠ (aa 2183) was modified Nterminally, either with a minimal histidine tag or with a homologous sequence of OprF. Both recombinant proteins were purified by nickel chelate affinity chromatography under native and denaturing conditions. Results:This produced three suitable candidates for a vaccination trial: protein HisFⅠ, which was purified in its native as well as in its refolded form, and the native purified Nterminally extended protein exFⅠ. In mice, significantly higher antibody titers and survival rates after challenge with P. aeruginosa were observed, following immunization with protein HisFⅠ purified under native conditions. Conclusion: A hybrid OprFOprⅠ molecule was cloned and a purification method which yields a protective vaccine against P. aeruginosa infections was established.