严重烧伤小鼠早期腹腔巨噬细胞CD14的动态变化

The dynamic observation of expression of macrophage CD14 in early stage of severely burned rats

目的：探讨烧伤后早期腹腔巨噬细胞（Ｍφ）ＣＤ１４分子的动态变化。方法：采用ＢＡＬＢ／ｃ小鼠２０％ＴＢＳＡⅢ度烧伤模型，分离和培养小鼠腹腔巨噬细胞，用免疫组织化学方法，观察了烧伤复苏组和延迟复苏组伤后１２ｈ内不同时间ＣＤ１４的变化。结果：烧伤复苏组和延迟复苏组在伤后１ｈ腹腔ＭφＣＤ１４表达就明显增加，复苏组、延迟复苏组阳性强度分别在伤后２，８ｈ达到峰值，这种改变在伤后１２ｈ内呈持续状态。从伤后８ｈ起，延迟复苏组小鼠上述改变均较立即复苏组明显。结论：在伤后过度炎症反应的发生发展中起重要作用的Ｍφ的激活及其反应性的增高，可能与烧伤引起ＣＤ１４的上调，从而使其增敏有关。

Objective: To observe the dynamic changes of CD14 in abdominal cavity macrophages in severely burned rats. Methods: BALB/c mice received 20%TBSA Ⅲ° burn injury were randomly divided into two groups: group A given fluid resuscitation instantly postburn and group B given delayed fluid resuscitation at 6 h postburn. Abdominal cavity macrophages at different intervals of time postburn were obtained in two groups for immunohistochemical analysis. Results: Significantly increased levels of CD14 were found at 1 h postburn in two groups. Group A peaked at 2 h and group B peaked at 8 h postburn in positive intensity .The changes remained at 12 h postburn. From 8 h postburn, every value was higher in the delayed resuscitation group than that in the immediate resuscitation group. Conclusion: Burn significantly increases the sensitivity in macrophages possibly because of upregulation of CD14.