TGF-β_1、TNF和维拉帕米对烧伤小鼠CD14表达的影响

Effects of TGF-β_1,TNF and verapamil on expression of CD14 mRNA in burned mice

目的：探讨转化生长因子β１（ＴＧＦ－β１）、肿瘤坏死因子（ＴＮＦ）和维拉帕米对严重烧伤小鼠早期腹腔巨噬细胞（Ｍφ）ＣＤ１４表达的影响。方法：ＢＡＬＢ／ｃ小鼠被随机地分成烧伤组和假烫组，将收集到的腹腔巨噬细胞分别加入不同浓度ＴＧＦ－β１（０．０２，０．２０，２．００μｇ／ｍｌ）、ＴＮＦ（０．１，１．０，１０．０μｇ／ｍｌ）和维拉帕米（１０，１００，１０００ｐｍｏｌ／ｍｌ）培养。用细胞原位杂交方法，观察ＴＧＦ－β１、ＴＮＦ和维拉帕米对小鼠ＭφＣＤ１４ｍＲＮＡ表达的影响。结果：（１）烧伤后腹腔ＭφＣＤ１４ｍＲＮＡ的表达明显增加。（２）０．０２，０．２０μｇ／ｍｌＴＧＦ－β１可使烧伤小鼠ＭφＣＤ１４ｍＲＮＡ表达增加，２μｇ／ｍｌ则可使烧伤小鼠ＭφＣＤ１４ｍＲＮＡ表达减少。ＴＮＦ、维拉帕米各浓度对烧伤小鼠ＭφＣＤ１４ｍＲＮＡ表达均无明显影响。结论：ＴＧＦ－β１能通过改变ＭφＣＤ１４ｍＲＮＡ的表达，调节其功能状态。ＴＮＦ。

Objective: To study the effects of TGF-β_1,TNF and verapamil on CD14 mRNA in peritoneal macrophages in severely burned mice in order to provide an experimental evidence for its further studies. Methods: Mouse were randomly divided into burn group and sham group. Mice peritoneal macrophages from burn group (n=5) or sham group (n=5) were isolated and incubated with TGF-β_1 (0.02,0.20,2.00 μg/ml), TNF(0.1,1.0,10.0 μg/ml) and verapamil(10,100,1000 pm/ml). The peritoneal macrophage CD14 mRNA expression was observed by in situ hybridization analysis. Results: (1) Significantly increased CD14 mRNA staining granules in cytoplasm were found in burn mice. (2) TGF-β_1 (0.02,0.20 μg/ml) enhanced the expression of CD14 in burned mice macrophages, and these effects could be reversed when the cells were treated with the highest dose. No difference was observed between macrophages from burned mice treated with TNF and verapamil and from burned mice untreated. Conclusion: TGF-β_1 can significantly change macrophage sensitivity possibly because of the changes of CD14 mRNA expression. These effects do not occur in burn mice incubated with TNF and verapamil. 