用平板计数和聚合酶链反应法检测湖水中大肠杆菌基因工程菌的稳定性

Detection of Stability of Genetically Engineered Escherichiacoli in Lake Water by Plate Counting and PolymeraseChain Reaction

用平板计数和聚合酶链反应（ＰＣＲ）法，检测了暴露于不同温度条件下自然湖水中大肠杆菌基因工程菌（ＭＭ３）的存活与衰亡规律。平板计数结果表明，４℃湖水中ＭＭ３的平均存活率为３５．３５％，２０℃为２２．１８％，３０℃为２０．１８％。ＰＣＲ检测指出，自然湖水温度的高低和暴露ＭＭ３周期的长短对检测结果没有影响。用含有氯霉素的ＬＢ平板，检测不同温度湖水中ＭＭ３的质粒稳定性。

おn this study, the survival and death rate of genetically engineered Escherichia coli (MM3) exposed in natural lake water at different temperature was detected by plate counting and polymerase chain reaction. The results of plate counting displayed that, the average survival rate of MM3 in lake water was 3535% at 4℃, and 2218% at 20℃,2018% at 30℃. The result of PCR showed that temperature of natural lake water and exposure time of MM3 didn't influence the detection results.Plasmid stability of MM3 in lake water at different temperature was detected on LB plates containing chloramphenicol, and plasmid loss wasn't found.