不同类型自毁性介质受体联合阻断对颈髓损伤的保护作用评价

Protective Effect of Combined Blockade of Various Autolesion Mediator Receptors on Cervical Cord Injury

目的探讨血小板活化因子（ｐｌａｔｅｌｅｔａｃｔｉｖａｔｉｎｇｆａｃｔｏｒ，ＰＡＦ）受体拮抗剂ＢＮ５２０２１阿片肽受体拮抗剂纳络酮联合应用对颈髓损伤后神经功能的协同保护作用。方法采用Ａｌｅｎ打击法造成猫Ｃ６段脊髓损伤，实验动物分为ＢＮ５２０２１组、纳络酮组、联合用药组及等渗盐水对照组，观察伤后颈髓组织血流量、血脊髓屏障、脊髓神经功能评分和病理组织学改变。结果联合用药组各项指标优于其他单独用药组。结论不同自毁性介质受体拮抗剂的联合应用将更加有效地抑制脊髓损伤后继发性损害的发生、发展，并改善脊髓的神经功能。

Aim To investigate the effects of combined blockade of platelet activating factor (PAF) receptor antagonist BN 52021 in combination with opiate receptor antagonist naloxone on neurological function and neurological tissue damage after cervical cord injury. Methods Spinal cord contusion at C 6 segment was made with Allen method in some experimental cats. The experimental animals were randomly divided into four groups: the saline group (the control group), the BN 52021 group, the naloxone group, and the combined administration group with BN 52021 and naloxone. The alteration of cervical cord blood flow, the blood spinal cord barrier permeability, the cervical cord tissue pathology and the neurological functional scores were inspected after cervical cord injury. Results The animals treated with BN 52021 or naloxone had significantly better functional scores than the controls at 6 weeks postinjury ( P <0.05). Moreover, the animals of combined administration showed significantly better neurologic recovery than that of either naloxone or BN 52021 treated animals ( P <0.05). The other indexes in combined administration animals were also superior to those of the naloxone or BN 52021 treated animals. Conclusion The combined administration of two kinds of autolesion mediators can more effectively prohibit the production and development of the secondary damage after cervical cord injury and improve spinal cord neurologic function.