内毒素、失血性休克大鼠线粒体质子跨膜转运和H^+-ATP酶的改变

Changes of Proton Translocation Across Inner Mitochondrial Membrane and H + ATPase in Endotoxic and Hemorrhagic Shock Rats

目的研究内毒素、失血性休克大鼠肝线粒体质子跨膜转运和Ｈ＋－ＡＴＰ酶的变化。方法大肠杆菌内毒素休克模型和失血性休克模型。采用荧光探针ＡＣＭＡ测定质子跨膜转运。结果（１）内毒素休克５小时亚线粒体在以ＡＴＰ、ＮＡＤＨ和ｓｕｃｃｉｎａｔｅ为底物时引起的ＡＣＭＡ最大荧光淬灭值显著减少（Ｐ＜０．０５）；最大荧光淬灭时间和半数荧光淬灭时间非常显著延长（Ｐ＜０．０１）。（２）内毒素休克早期，线粒体Ｈ＋－ＡＴＰ酶活性显著升高（Ｐ＜０．０５），晚期非常显著下降。（３）内毒素休克大鼠肝线粒体膜结合ＰＬＡ２、血浆和线粒体ＭＤＡ升高（Ｐ＜０．０５）。（４）失血性休克时Ｈ＋－ＡＴＰ酶和质子转运无显著改变。结论内毒素休克时线粒体跨膜质子转运和Ｈ＋－ＡＴＰ酶活性显著下降。

Aim To investigate the changes of proton translocation across the inner mitochondrial membrane (IMM) and H + ATPase of hepatocytes in endotoxic and hemorrhagic shock rats. Methods Experiments were performed on 100 E Coli endotoxic and hemorrhagic shock rats. The changes of proton translocation were studied with fluorescent probe of ACMA. Results (1) Five hours after LPS injection, the fluorescent quenching of ACMA after adding ATP, NADH and succinate significantly decreased ( P <0.05). The time of maximum fluorescent quenching and the half time of fluorescent quenching were significantly prolonged ( P <0.01). (2) The mitochondrial H + ATPase activity significantly increased in the early stage of endotoxic shock ( P <0.01). (3) The mitochondrial membrane associated PLA 2 activity, plasma MDA and mitochondrial MDA content significantly increased in endotoxic shock rats ( P <0.05). (4) There were no significant changes of H + ATPase and proton translocation in hemorrhagic shock rats. Conclusion It indicates that proton translocation across the IMM and mitochondrial H + ATPase activity significantly decrease in endotoxic shock, and there were no significant change in hemorrhagic shock.