酶催化法进行超氧自由基(O_2^(·-))动力学检测

A Dynamical Examination of Supeioxide Radicol (O_2^(·-)) Through Enzyme Reaction

实验选择黄嘌呤──黄嘌呤氧化酶作为系统，反应在Ｏ２存在的条件下，废物黄嘌呤发生催化氧化，产生超氧自由基（Ｏ２．－），由２．６－二氯靛酚钠试剂检测。通过运用Ｍｉｃｈａｅｌｉｓ反应机理处理，最终得到米氏常数（ＫＭ）。Ｃｕ２＋能抑制黄嘌呤氧化酶的作用效果，其抑制作用可通过ｖ０－［Ｃｕ２＋］曲线看出。

Xanthine-xanthine oxidase is chosen as system for this experment. The reaction is a catalytical exidation of the supstiate xanthine in the presence of O2, the generated supevoxide raclical (O2.-) is examined by the 2, 6 -Dichloroindophenol sodium. The Michaclis constant then can be finally concluded through mechanism treatment of Michaelis reaction. Cu2+ can inhibit the effeet of xanthine oxidase. The inhibition can be obseived from v0[Cu2+] curve.