理化因素对新疆紫草培养细胞紫草宁形成的影响

Effect of physical and chemical factors on shikonin formation in the cultured cells of Arnebia euchroma . Journal of Zhejiang Agricultural University

研究了在促进剂Ｃｕ２＋和抑制剂２，４－Ｄ及光的作用下，对产色素培养细胞紫草宁形成的影响。对４，１５，２５，３５和４５ｄ的紫草培养细胞的电镜观察和细胞超微结构分析的结果表明，１５ｄ加促进剂Ｃｕ２＋的新疆紫草培养细胞形成的色素颗粒（紫草宁）最多，４ｄ和加抑制剂２，４－Ｄ的培养细胞中均无明显的色素颗粒。

The effect of promoter Cu 2+ , restrainer 2,4 D and light on sikonin formation in pigmented cultured cells of Arnebia euchroma were investigated. The ultrastructural observation of the cultured cells at 4, 15, 25, 35, 45 days by electronic microscope show that pigment granules were the most in the cultured cells of 15 days plus promoter Cu 2+ , and there are not pigment granules in the cultured cells of 4 days or plus restrainer 2,4 D.