摘要
棉铃虫Helicoverpaarmigera中肠羧酸酯酶(CarEs)比活力随幼虫发育阶段呈有规律的变化。5日龄以前CarEs比活力增长比较缓慢,5日龄以后CarEs比活力快速增长,8日龄开始CarEs比活力下降,直到预蛹期,CarEs比活力又开始上升。用聚丙烯酰胺凝胶电泳分离到的9条同功酶带中,E2、E4、E5、E6、E7和E9对β乙酸萘酯(βNA)的水解活性高于对α乙酸萘酯(αNA),E1、E3、和E8对上述两种底物的水解活性正好相反,对αNA活性高于对βNA。低剂量的对硫磷、马拉硫磷、倍硫磷、增效磷(SV1)、灭多威和溴氰菊酯对棉铃虫中肠CarEs比活力和对底物的亲和力具有明显的影响。药剂处理后48h,CarEs的比活力明显降低,对底物的亲和力则与药剂的种类有关。马拉硫磷处理后48h,在观察到的6条主要同功酶带中,E3的活性明显降低,其次是E6、E2和E1;而E5的活性则明显升高,E4的活性也有所升高。用含001%的芸香苷、2十三烷酮和槲皮素的人工饲料饲养棉铃虫经2代或7代后,CarEs比活力均明显升高。
Induction of carboxylesterase (CarEs) by insecticides and plant allelochemicals and developmental changes of CarEs were investigated during 1992~1996 in cotton bollworms, Helicoverpa armigera (Hübner). The specific activities of midgut CarEs increased slowly before 5th day and then rose rapidly in larvae. The specific activity started to reduce from 8th day larvae to prepupae and then rose again. E2, E4, E5, E6, E7 and E9 activities in nine isoenzyme bands obtained by polyacrylamide gel electrophoresis were higher using β naphthyl acetate than α naphthyl acetate as substrate, whereas E1, E3 and E8 activities were reversed. Parathion, malathion, fenthion, SV1, methomyl and deltamethrin at sublethal dosage made the total activities of CarEs reduce, but malathion induced the activities of E4 and E5 in six isoenzyme bands at 48h after treatment by malathion and depressed the activities of E1, E2, E3 and E6. Plant allelochemicals, rutin, quercetin and 2 tridaconone, can significantly induced CarE activities of larvae.
出处
《昆虫学报》
CAS
CSCD
北大核心
1998年第S1期7-13,共7页
Acta Entomologica Sinica
基金
国家自然科学基金
关键词
棉铃虫
羧酸酯酶
杀虫药剂
植物次生性物质
Helicoverpa armigera , carboxylesterase, insecticide, plant allelochemical