松毛虫赤眼蜂和螟黄赤眼蜂羧酸酯酶性质比较研究

A PRELIMINARY STUDY ON THE PRORERTIES OF CARBOXYLESTERASE IN TRICHOGRAMMA DENDROLIMI AND T CHILONIS

该文对松毛虫赤眼蜂Ｔｒｉｃｈｏｇｒａｍｍａｄｅｎｄｒｏｌｉｍｉ和螟黄赤眼蜂Ｔｃｈｉｌｏｎｉｓ羧酸酯酶的毒理学和生物化学性质进行了初步研究。由时间进程曲线确定了赤眼蜂羧酸酯酶活性测定中的最适反应时间为４０ｍｉｎ。对两种赤眼蜂单头羧酸酯酶活性的测定表明，松毛虫赤眼蜂羧酸酯酶活性主要分布在０～１ＯＤ／（ｍｇ·ｍｉｎ）之间，而螟黄赤眼蜂羧酸酯酶活性主要分布在１～４ＯＤ／（ｍｇ·ｍｉｎ）之间。比较两种赤眼蜂羧酸酯酶米氏常数（Ｋｍ）值，螟黄赤眼蜂羧酸酯酶对底物的亲和力比松毛虫赤眼蜂羧酸酯酶对底物的亲和力高。对氧磷对螟黄赤眼蜂和松毛虫赤眼蜂羧酸酯酶的抑制作用没有明显差异，而对氧磷对松毛虫赤眼蜂羧酸酯酶的抑制作用明显强于磷酸三苯酯（ＴＰＰ）。该文还将棉铃虫Ｈｅｌｉｃｏｖｅｒｐａａｒｍｉｇｅｒａ（Ｈüｂｎｅｒ）羧酸酯酶部分性质与两种赤眼蜂作了比较。

The biochemical and toxicological properties of carboxylesterase (CarE) were investigated in Trichogramma dendrolimi and T chilonis The optimal reaction time of CarE with α NA was 40 minutes The CarE activity mainly distributed in 0～1 OD/(mg·min) in T dendrolimi and 1～4 OD/(mg·min) in T chilonis respectively The comparison of Michaelis constants ( K m) of CarE between T dendrolimi and T chilonis indicated that the affinity of the latter to substrate was stronger than that of the former There was no significant difference of CarE inhibition between T dendrolimi and T chilonis when used paraoxon as inhibitor The inhibition of paraoxon to the carboxylesterase of T dendrolimi was much stronger than that of TPP