胃癌活检标本中p53基因突变的研究

Research on p53 gene mutation of biopsy samples from stomach cancer patients

目的探索胃癌癌变过程中抑癌基因p53的变异规律．方法用聚合酶链反应，单链构象多态性，银染方法，检测胃癌活检标本30例（男20例，女10例，平均年龄56．8岁）的p53基因突变．结果病理诊断为胃癌的标本中检出的30例p53基因外显子5～8的突变18例，阳性率为60％．其癌基因的扩增和重排多发生于胃癌进展期，而p53基因的点突变发生于癌变各期结论胃癌癌变是多基因异常累积所引起的渐进过程，基因改变的数目。

AIM To diagnose the alteration of oncogenes and tumor suppressor genes in gastric carcinogenes.METHODS From the reaction of polymerase,polymorphism of single strand contormation and, by using the silver staining, we have carefully examined p53 gene mutatioo of biopsy samples among 30 stomach cancer petients.RESULTS The mutation rate of P53 in exon 5 8 was 18/30 (60.0%). Point mutation of p53 was bond at both early and edvaned tumors. In contrast amplification of oncogenes and loss of tumor suppressor genes were lorratated with poorly differentiated and metaststic tumors.CONCLSION Gastric carcinogenesis is a gradually developed process, which results from sequencial alteration of meltipenes. The malignant phenotype is associated with the degree of genetic abnormality.