慢性乙型肝炎患者外周血单个核细胞内HBV DNA存在的意义

HBV DNA in peripheral blood mononuclear cells of patients with chronic hepatitis B

目的探讨外周血单个核细胞（PBMC）内HBVDNA与HBV标志物的关系及在慢性肝病发展过程中的作用．方法应用RCR技术结合斑点杂交技术测定血清HBVDNA及PBMC内HBVDNA血清学标志物采用ELISA法结果在HBsAg＋，HBeAg十组48例中，血清与PBMC内HBVDNA阳性检出率分别为93．8％和83．3％；在HBsAg＋，抗-HBe+组41例中，其阳性检出率分别为48．8％和58．5％.两组间存在显著性差异（P＜0．01）在其他各组中血清及PBMC内HBVDNA检出率均较抵有6例呈单纯PBMC内HBVDNA阳性.在不同类型慢性乙肝患者中，CSH和CMoH组PBMC-HBVDNA检出率分别为78．6％和61．8％，与CMiH组（25．0％）、ASC组（14．3％）比较，存在非常显著性差异（P＜0．01），呈随病情加重而检出率增高的趋势结论PBMC清除HBVDNA较血液缓慢，在病毒持续感染及造成肝细胞损伤过程中可能起一定作用．

AIM To study the value of HBV DNA in Peripheral blood mononuclear cells (PBMC) of patients with HBV markers and in the process of chronic bopetitis B.METHODS HBV DNA in serum and PBMC was dethcted by PCR and dot blot methods. Serum HBV markers were detected by ELISA methods.RESULTS In 48 cases of HBsAg + & HBeAg +, the positive rates of HBV DNA in serum and PBMC were 93. 8%and 83 .3% respetively. In 41 cases of HBsAg+ & antiHBe+, the positive rates were 48.8% and 58.5%. There was a markedly difference between them (P< 0 .01 ). The positive rates were lower in other groups. HBV DNA was only found in PBMC of 6 patients. The positive rates of PBMC-HBV DNA in CSH and CMoH were 78.6% and 61 .8%,significantly higber than that in CMiH (25 .0% ) and in ASC (14. 3%' P<0.01). The peitive rates tended to be higher with the proces of severity of wepetitis B.CONCLUSION HBV DNA was got rid of more slowly in PBMC than that in serum. It might result in persistent infection of HBV to hepatic cells.