VEGF在甲状腺癌及转移淋巴结中的表达及与DNA倍体、AgNOR的关系

CORRELATION OF THE EXPRESSION OF VEGF,DNA AND AgNOR IN THYROID CARCINOMA

目的研究血管内皮生长因子（VEGF）在甲状腺癌及其转移淋巴结中的表达及与癌细胞DNA倍体、核仁形成区（AgNOR）的关系。方法应用免疫组织化学SABC法检测136例甲状腺癌及淋巴结中VEGF的表达，同时进行细胞DNA含量及AgNOR自动图像分析检测，10例正常甲状腺组织作对照。结果10例正常甲状腺组织VEGF（一），136例甲状腺癌组织中VEGF阳性表达72．79％（99／136），淋巴结中VEGF阳性表达97％（132／136）。DNA倍体平均值及AgNOR计数5年以上生存组病例明显低于5年以内死亡组（P＜O．01，P＜O．05）。结论检测VEGF、DNA倍体及Ag－NOR有助于评估甲状腺癌的生物学行为及预后。

Objective: To study the relationship of VEGF, DNA and AgNOR in human thy-roid carcinoma and the metastasis of lymphaden. Methods: The expression of VEGF, DNA and Ag-NOR were stuided by immunohistochemistry and image analysis system. Results: On the contrary,the expression of VEGF was negative in 10 case of thyroid tissue. The positivity rates of VEGF were72. 79 %, 97 % in 136 case of thyroid carcinoma and in the metastasis of lymphaden respectively. Theaverage value of DNA and AgNOR number in more than 5 years Survival groups was lower than inless than 5 years death groups (P ＜ 0. o1, P ＜ 0. 05 ). Conclusion: Examining the expression ofVEGF, DNA and AgNOR may be avilable to estimate the patients prognosis.