槲皮素对原代培养的牛肺动脉平滑肌细胞增殖的抑制作用

Inhibitory Effect of Quercetin on Primary Cultural Calf Pulmonary Artery Smooth Muscle Cell Proliferations

目的:研究酪氨酸蛋白激酶抑制剂槲皮素对肺动脉平滑肌细胞增殖的影响。方法:用原代培养的小牛肺动脉平滑肌细胞,采用 MTT 比色法、流式细胞术、细胞计数、Giemsa染色对肺动脉平滑肌细胞增殖的影响进行观察。结果:与对照组相比,槲皮素处理组可显著地抑制肺动脉平滑肌细胞的增殖,而且主要是细胞周期的 G_0/G_1到 S 期受抑制。结论:槲皮素可显著地抑制肺动脉平滑肌细胞的增殖,其机制可能是其对酪氨酸蛋白激酶的抑制作用。

Objective:To explore the effect of tyrosine protein kinases(TPK)inhibitor quercetin on pulmonary artery smooth muscle cells(PASMC)proliferation.Methods:By primary culture of calf PASMC,the effect of PASMC proliferation was analysed using MTT colorimetry,flow cytometry,Giemsa staining and cell counts.Results:As compared with control group,PASMC proliferations in quercetin group were significantly inhibited from G_0/G_1 to S phases in the cell cycle. Conclusions:TPK inhibitor quercetin could significantly inhibit PASMC proliferation.The mechanism that quercetin in- hibited PASMC proliferation might be from inhibiting TPK.