慢支固本冲剂抗呼吸道感染的实验研究

Experimental Study on Anti-inflammation Effect of Manzhi Guben Granule(慢支固体冲剂)on Respiratory Tract of Rats and Mice

目的:以中医扶正祛邪理论为指导,进行补益中药抗呼吸道感染的实验研究。方法:应用慢支模型鼠,经气道吸入法感染绿脓杆菌,并在设定的条件下,(1)取实验动物气管组织制成匀浆作细菌定量培养;(2)取气管组织作病理切片,检查粘膜上皮的病理变化;(3)取气管组织作扫描电镜检查,观察粘膜上皮超微结构及细菌粘附于粘膜上皮的情况,据此研究绿脓杆菌感染机理及中药慢支固本冲剂的治疗作用。结果:绿脓杆菌不能粘附于正常鼠完整的气道粘膜,而可粘附于慢支模型鼠病变的粘膜上皮,并定居、繁殖造成长期带菌。结论:给服慢支固本冲剂,能使病变粘膜上皮复愈,从而抵抗绿脓杆菌的粘附。

Objective:Under the guidance of traditional Chinese medical theory of improving the general condition to eliminate pathogens,to study the effects and mechanism of Manzhi Guben Granule(MZGBG),a Chinese tonifying drug in pre- venting respiratory infection.Methods:Chronic bronchitis model rats and mice infected with inhalational pseudomonas aeruginosa were treated with MZGBG,and examinations were done on:(1)Quantitative determination of bacteria in bronchial tissue culture;(2)Pathologic change of epithelial mucous membrane of trachea and(3)Scanning electron mi- croscopic observation on ultrastructure of tracheal mucous epithelium and its condition of bacteria adhering.And the results were compared with those of non-infected animals.Results:Pseudomonas aeruginosa couldn't adhere to the intact mucous membrane of normal rats and mice,hut could adhere to,settle down and multiply on the infected mucous membrane ep- ithelia,and thus results in a state of long-term bacteria carrier.MZGBG could reduce the degree of pathologic changes of trachea.Conclusion:MZGBG could protect tracheal mucous membrane from bacteria adherence by repairing the pathologic changes.