延肾胶囊延缓残肾损伤机理的实验研究

The Experiment Study on the Mechanism of Retarding Remnant Kidney Injury by Yanshen Capsule(延肾胶囊)

目的:探讨延肾胶囊对慢性肾功能衰竭的治疗作用及其机理。方法:在5/6肾切除大鼠慢性肾衰动物模型上,以尿毒清冲剂和保肾康片为对照,观察延肾胶囊对实验动物残肾组织和残肾功能的保护作用及有关机理指标的变化。结果:给予延肾胶囊治疗的5/6肾切除大鼠,术后120天时与未治疗的5/6肾切除大鼠相比,残肾组织总自由基含量显著回降,残肾组织超氧化物歧化酶和过氧化氢酶活性及维生素 E 含量显著回升,外周血单个核细胞内游离钙浓度和白介素-1产量回降,残肾组织羟脯氨酸含量回降,肾小球硬化比率和肾间质纤维化程度降低,血浆肌酐、血浆尿素氮、平均动脉压、红细胞压积、尿蛋白等反应肾功能的指标显著改善。与尿毒清冲剂和保肾康片相比,延肾胶囊的抗氧化作用更强,对残肾的保护作用更好。结论:延肾胶囊能通过抑制残肾组织过高的氧自由基反应、降低细胞内游离钙浓度、减少炎性细胞的白介素-1产生量,而抑制残肾纤维化,保护残肾功能,减缓残肾的进行性损伤,其疗效优于尿素清冲剂和保肾康片,具有较好的开发价值。

Objective:To observe the effect and mechanism of Yanshen Capsule (YSC) for chronic renal failure (CRF).Meth- ods:Rat models with chronic renal failure induced by nephrectomizing 5/6 of the kidney were treated with YSC;the re- nal function,histology,and other indexes reflecting the effect and its mechanism were observed to evaluate the effect of YSC and compared with those of another two traditional Chinese drugs,Niaoduqing granule (尿毒清冲剂) and Baoshenkang tablet (保肾康片).Results:In rats treated with YSC,accompanied by the reduction of oxygen free radical reaction,the free calcium concentration in peripheral blood mononuclear cells and the interleukin-1 production from these cells depressed markedly,the oxyproline content of renal tissue reduced,the sclerotic glomerular proportion and the extent of renal mesenchymal fibrosis decreased significantly,and renal function improved.The YSC produced better curative ef- fect than Niaoduqing granule and Baoshenkang tablet did.Conclusion:YSC could retard the progress of remnant kidney injury through its action of suppress oxygen free radical reactions,depress cytosolic free calcium,reduce interleukin-1 pro- duction,etc.